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Published on: February 9, 2024
Micropillar enhanced FRET-CRISPR biosensor for nucleic acid detection
Mengdi Bao1, Stephen J Dollery2, Fnu Yuqing1
1Department of Chemical and Environmental Engineering, University of California, Riverside, CA, USA.
This study enhances CRISPR pathogen detection using micropillars for higher sensitivity. The novel approach boosts HPV 16 DNA detection tenfold, improving microfluidic biochemical reactions.
Area of Science:
- Biotechnology
- Molecular Biology
- Microfluidics
Background:
- CRISPR technology offers high sensitivity and specificity for pathogen detection.
- High-aspect-ratio microstructures can enhance biochemical applications, but their use in CRISPR detection is underexplored.
Approach:
- Developed a FRET-based biosensor combined with high-aspect-ratio microstructures and Cas12a trans-cleavage for HPV 16 DNA detection.
- Investigated surface chemical treatments to improve FRET assay performance.
- Created a method to reduce bubble generation in microfluidic devices.
Key Points:
- Micropillars with higher density significantly improved molecular binding.
- Detection sensitivity for HPV 16 DNA fragments increased tenfold.
- Optimized surface treatments and microfluidic bubble mitigation.
Conclusions:
- Introduced a novel micropillar-based approach for CRISPR-enabled viral detection.
- Demonstrated enhanced sensitivity and optimized microfluidic conditions for biochemical assays.
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