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Updated: Jul 17, 2025
Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
Published on: September 18, 2020
Identification of Potential Abnormal Methylation-Modified Genes in Coronary Artery Ectasia
Xiuchun Yang1, Yijun Zong2, Zhentian Zhang1
1Department of Cardiology, The Second Hospital of Hebei Medical University, Shijiazhuang, China.
Insights
This study identifies abnormal methylation-modified genes in coronary artery ectasia (CAE) patients. These genes, linked to signaling pathways, offer new insights into CAE pathogenesis and potential therapeutic targets.
Area of Science:
- Cardiovascular Research
- Epigenetics
- Molecular Biology
Background:
- Coronary artery ectasia (CAE) is a recognized anatomical abnormality.
- The underlying causes and molecular mechanisms of CAE remain largely unknown.
Purpose of the Study:
- To identify genes with abnormal DNA methylation in patients diagnosed with coronary artery ectasia.
- To establish a foundational understanding for future research into CAE pathogenesis.
Main Methods:
- RNA sequencing was performed on peripheral blood samples from CAE patients to identify differentially expressed genes (DEGs).
- DNA methylation profiles (GSE87016) were analyzed to identify differentially methylated genes (DMGs).
- Integration of DEGs and DMGs, followed by protein-protein interaction analysis and RT-PCR validation, identified core abnormal methylation-modified genes.
Main Results:
- 152 DEGs and 4318 DMGs were identified.
- Nine down-regulated DEGs (hypermethylation) and 11 up-regulated DEGs (hypomethylation) were found in CAE patients.
- Ten core abnormal methylation-modified genes were identified, including specific genes like netrin G1 and adrenomedullin, implicated in pathways such as cell adhesion and the renin-angiotensin system.
Conclusions:
- Abnormal methylation-modified differentially expressed genes are associated with coronary artery ectasia.
- These epigenetically altered genes, particularly those involved in key signaling pathways, may play a significant role in the development of CAE.
Background:
Coronary artery ectasia (CAE) is an easily recognized abnormality of coronary artery anatomy and morphology. However, its pathogenesis remains unclear.
Objectives:
This study aimed to identify abnormal methylation-modified genes in patients with CAE, which could provide a research basis for CAE.
Methods:
Peripheral venous blood samples from patients with CAE were collected for RNA sequencing to identify differentially expressed genes (DEGs), followed by functional enrichment. Then, the DNA methylation profile of CAE was downloaded from GSE87016 (HumanMethylation450 BeadChip data, involving 11 cases and 12 normal controls) to identify differentially methylated genes (DMGs). Finally, after taking interaction genes between DEGs and DMGs, abnormal methylation-modified genes were identified, followed by protein-protein interaction analysis and expression validation using reverse transcriptase polymerase chain reaction.
Results:
A total of 152 DEGs and 4318 DMGs were obtained from RNA sequencing and the GSE87016 dataset, respectively. After taking interaction genes, 9 down-regulated DEGs due to hypermethylation and 11 up-regulated DEGs due to hypomethylation were identified in CAE. A total of 10 core abnormal methylation-modified genes were identified, including six down-regulated DEGs due to hypermethylation (netrin G1, ADAM metallopeptidase domain 12, immunoglobulin superfamily member 10, sarcoglycan dela, Dickkopf WNT signaling pathway inhibitor 3, and GATA binding protein 6), and four up-regulated DEGs due to hypomethylation (adrenomedullin, ubiquitin specific peptidase 18, lymphocyte antigen 6 family member E, and MX dynamin-like GTPase 1). Some signaling pathways were identified in patients with CAE, including cell adhesion molecule, O-glycan biosynthesis, and the renin-angiotensin system.
Conclusions:
Abnormal methylation-modified DEGs involved in signaling pathways may be involved in CAE development.
