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Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
Published on: May 8, 2013
fbl-typing and Antimicrobial Resistance Analysis of Staphylococcus lugdunensis
Gang Chen1, Yue Hou2, Liyan Ye1
1Department of Clinical Laboratory, First Medical Center, Chinese People's Liberation Army General Hospital, 100853 Beijing, China.
Background:
A broad variety of infections, ranging from skin infections to infective endocarditis can be caused by Staphylococcus lugdunensis. Bacterial virulence is often related to virulence genes, so we sought to investigate the relationship between virulence genes and the pathogenicity of S. lugdunensis and to explore an appropriate typing method to distinguish different pathogenic phenotypes of S. lugdunensis.
Methods:
We describe the distribution of several virulence genes in different infection types in an attempt to find the relationship between virulence genes and pathogenicity. Subsequently, we make the Matrix-Assisted Laser Desorption/Ionization-Time-of-Flight Mass Spectrometry (MALDI-TOF MS) dendrogram and fbl-typing were performed using BioNumerics software, tried to compare the correlation between different methods and the different infectious diseases, and antimicrobial resistance of the strains, in order to obtain the epidemic characteristics and antimicrobial resistance information of S. lugdunensis based on a molecular approach.
Results:
The results of virulence genes showed that the seven virulence genes we have described existed in most strains, and there was no significant correlation between virulence gene distribution and infection type. Compared with the MALDI-TOF MS dendrogram, we found that fbl-typing could better correspond to the pathogenic phenotype, with better recognition and reproducibility. In the phylogenetic tree constructed in the fbl R-region, we found a tendency for some infection types to be distributed in clusters, new type 3 was the most dominant fbl-type, followed by fbl47b. Bone and joint infection isolates and ear infection isolates were significantly clustered together, in addition, all the oxacillin-resistant isolates were concentrated in fbl-type fbl45j and fbl47b.
Conclusions:
In this study, we found no significant correlation between virulence genes from S. lugdunensis isolates and the site of infection. The fbl-typing has the characteristics of convenient operation, low cost, high repeatability, and is preferable to indicate the pathogenic phenotype. Based on fbl-typing, we described the epidemiological characteristics of S. lugdunensis in a hospital and supplemented the data for fbl-typing. We recommend that fbl-typing method be extended and supplemented.
Insights
Virulence genes in Staphylococcus lugdunensis do not correlate with infection type. Fbl-typing is a reliable method for determining pathogenic phenotypes and epidemiological characteristics of S. lugdunensis infections.
Area of Science:
- Medical Microbiology
- Bacterial Pathogenesis
- Molecular Epidemiology
Background:
- Staphylococcus lugdunensis causes diverse infections, from skin issues to severe endocarditis.
- Bacterial virulence is often linked to specific virulence genes.
- Understanding these genes and typing methods is crucial for managing S. lugdunensis infections.
Purpose of the Study:
- Investigate the relationship between virulence genes and S. lugdunensis pathogenicity.
- Explore an effective typing method to differentiate pathogenic phenotypes.
- Determine epidemic characteristics and antimicrobial resistance patterns.
Main Methods:
- Analyzed the distribution of seven key virulence genes across different infection types.
- Utilized Matrix-Assisted Laser Desorption/Ionization-Time-of-Flight Mass Spectrometry (MALDI-TOF MS) dendrograms.
- Performed fbl-typing using BioNumerics software for comparative analysis.
Main Results:
- No significant correlation was found between the distribution of the seven studied virulence genes and infection type.
- Fbl-typing demonstrated superior correlation with pathogenic phenotypes compared to MALDI-TOF MS dendrograms.
- Specific fbl-types clustered certain infection types (e.g., bone/joint, ear), and oxacillin resistance was linked to fbl-types fbl45j and fbl47b.
Conclusions:
- Virulence gene presence in S. lugdunensis did not correlate with infection site.
- Fbl-typing is a convenient, cost-effective, and reproducible method for identifying pathogenic phenotypes.
- Fbl-typing provides valuable epidemiological insights into S. lugdunensis, recommending its broader application and supplementation.
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