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Updated: Jul 17, 2025

Author Spotlight: Exploring Cellular Zinc Regulation Through ZnT1 Functionality
Published on: June 2, 2023
Internalization and trafficking of zinc transporters
1Department of Medical Genetics & Cell Biology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, P.R. China.
Abstract:
In huma, two transporter families, the zinc transporters (ZNT/solute carrier 30 family [SLC30A]) and the Zrt- and Irt-like proteins (ZIP/solute carrier 39 family [SLC39A]), play vital roles in maintaining zinc homeostasis. ZIPs could increase the concentration of cytosolic Zn2+ by importing zinc from the extracellular environment or organelles into the cytosol, while ZnTs work in the opposite direction as they mediate the export of zinc from the cytosol into organelles or out of the cells. Mammalian cells express 10 ZnT exporters and 14 ZIP importers, and zinc or other transition metal ions may modulate their gene expression. The localization and post-translational trafficking of zinc transporters within the cells are often controlled in response to varying zinc concentrations, which likely impact the regulation of cellular zinc homeostasis. This chapter briefly summarizes the progress made on the intracellular trafficking of ZIPs and outlines the protocols used to study the endocytosis and trafficking of a representative human zinc transporter, ZIP4.
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