Related Experiment Video
Updated: Jul 9, 2026
08:22
IR-TEx: An Open Source Data Integration Tool for Big Data Transcriptomics Designed for the Malaria Vector Anopheles gambiae
Published on: January 15, 2020
6.2K
Deltamethrin and transfluthrin select for distinct transcriptomic responses in the malaria vector Anopheles gambiae
Marius Gonse Zoh1,2, Jean-Marc Bonneville3, Frederic Laporte3
1Laboratoire d'Ecologie Alpine (LECA), Grenoble-Alpes University, Savoie Mont-Blanc University, CNRS, 38041, Grenoble, France. marius_zoh@yahoo.com.
Malaria Journal
|September 4, 2023
Summary
Pyrethroid insecticides like deltamethrin and transfluthrin rapidly select for resistance in Anopheles mosquitoes. Unexpectedly, transfluthrin also induces broad metabolic resistance, impacting malaria control strategies.
Area of Science:
- Entomology
- Molecular Biology
- Public Health
Background:
- Widespread pyrethroid use drives insecticide resistance in African Anopheles mosquitoes.
- New active ingredients are crucial for effective vector control.
- Transfluthrin, a polyfluorinated pyrethroid, was investigated for its potential to manage resistance.
Purpose of the Study:
- To compare resistance dynamics between transfluthrin and deltamethrin in Anopheles gambiae.
- To investigate the molecular mechanisms of resistance development.
- To assess transfluthrin's efficacy in managing pyrethroid resistance.
Main Methods:
- Controlled selection experiment over 33 generations using deltamethrin and transfluthrin.
- Monitoring of resistance levels and L1014F kdr mutation frequency.
- Transcriptomic analysis (RNA-seq) to identify gene expression variations.
Main Results:
- Rapid resistance increase to both insecticides, linked to kdr mutation.
- Transfluthrin selection induced a broader transcriptional response than deltamethrin.
- Over-transcription of detoxification genes (CYP6M2, CYP9K1, CYP6AA1) observed in transfluthrin-selected mosquitoes.
Conclusions:
- Recurrent pyrethroid exposure rapidly selects for diverse resistance mechanisms.
- Transfluthrin selects for metabolic resistance, including P450s, contrary to expectations.
- Further research on mosquito adaptive responses to new insecticides is vital for malaria control programs.
