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Updated: Jun 17, 2026

Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications
Published on: September 15, 2017
Influence of poly-L-ornithine-bile acid nano hydrogels on cellular bioactivity and potential pharmacological
Bozica Kovacevic1, Melissa Jones1, Susbin Raj Wagle1
1The Biotechnology & Drug Development Research Laboratory, Curtin Medical School & Curtin Health Innovation Research Institute, Curtin University, Bentley, Perth, WA 6102, Australia.
Abstract:
Aim: Cellular bioactivity and pathophysiological changes associated with chronic disorders are considered pivotal detrimental factors when developing novel formulations for biomedical applications. Methods: This paper investigates the use of bile acids and synthetic polypeptide poly-L-ornithine (PLO) in formulations and their impacts on a variety of cell lines, with a particular focus on their cellular bioactivity. Results: The hepatic cell line was the most negatively affected by the presence of PLO, while the muscle and beta-pancreatic cell lines did not show as profound of a negative impact of PLO on cellular viability. PLO was the least disruptive regarding mitochondrial function for muscle and beta cells. Conclusion: The addition of bile acids generally decreased mitochondrial respiration and altered bioenergetic parameters in all cell lines.
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