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Updated: Jul 17, 2025

Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy
Published on: February 7, 2022
`Cryo-EM': electron cryomicroscopy, cryo electron microscopy or something else?
Richard Henderson1, Samar Hasnain2
1MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, United Kingdom.
Abstract:
Structural biology continues to benefit from an expanding toolkit, which is helping to gain unprecedented insight into the assembly and organization of multi-protein machineries, enzyme mechanisms and ligand/inhibitor binding. During the last ten years, cryoEM has become widely available and has provided a major boost to structure determination of membrane proteins and large multi-protein complexes. Many of the structures have now been made available at resolutions around 2 Å, where fundamental questions regarding enzyme mechanisms can be addressed. Over the years, the abbreviation cryoEM has been understood to stand for different things. We wish the wider community to engage and clarify the definition of cryoEM so that the expanding literature involving cryoEM is unified.
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