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Related Experiment Video

Updated: Jul 17, 2025

Fast Inspection of Quality of Indigo Naturalis by Multiple Light Scattering
03:40

Fast Inspection of Quality of Indigo Naturalis by Multiple Light Scattering

Published on: August 18, 2023

487

High-Quality Indigo Naturalis Obtained with Automatic Foam Separation.

Xin Yang1, Jun Tang1, Juan Su1

  • 1State Key Laboratory of Southwestern Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, PR China.

ACS Applied Materials & Interfaces
|September 5, 2023
PubMed
Summary

Foam-separation technology enhances Indigo Naturalis production, yielding higher active ingredient concentrations and reduced skin irritation. This method improves efficiency and quality for modern industrial applications.

Keywords:
Indigo Naturalisbiological activityfoam separationindustrial manufacturequality optimization

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Last Updated: Jul 17, 2025

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Area of Science:

  • Herbal Medicine
  • Biotechnology
  • Chemical Engineering

Background:

  • Indigo Naturalis, an ancient plant dye, possesses valuable medicinal properties including antibacterial, anti-inflammatory, and anticancer effects.
  • Traditional manual stirring methods for Indigo Naturalis extraction are inefficient, labor-intensive, and yield unstable quality and low output, hindering industrial scalability.

Purpose of the Study:

  • To enhance the industrial applicability of high-quality Indigo Naturalis using foam-separation technology.
  • To optimize foam-separation process parameters for improved active ingredient content, reduced skin irritation, and enhanced antioxidative stress activity.

Main Methods:

  • Foam-separation technology was employed to refine Indigo Naturalis.
  • Process parameters, including liquid level difference and foaming intensity, were optimized.
  • Optimized parameters were evaluated based on active ingredient content, skin irritation, and antioxidative stress activity.

Main Results:

  • Optimized foam separation (40 cm liquid level difference, 0.35 MPa foaming intensity) significantly increased indigo (1.6x) and indirubin (3x) content.
  • Purified Indigo Naturalis showed reduced total ash content (86% to 70%) and lower pH (12.18 to 9.71).
  • Animal studies confirmed significantly reduced skin irritation and enhanced antioxidative stress activity (p < 0.01) post-foam separation.

Conclusions:

  • Foam-separation technology effectively refines active ingredients in Indigo Naturalis, improving production efficiency and product quality.
  • The optimized process yields a purer, less irritating, and more potent form of Indigo Naturalis suitable for industrial use.