MIL-CELL: a tool for multi-scale simulation of yeast replication and prion transmission

Area of Science:

• Yeast genetics and prion biology
• Computational biology and mathematical modeling

Background:

• Saccharomyces cerevisiae harbors amyloid-based prions like [URE3], [PSI+], and [PIN+].
• Prion acquisition and loss in yeast involve spontaneous nucleation, cell division transmission, amyloid dissolution, and preferential segregation.

Purpose of the Study:

• To develop a computational tool, MIL-CELL, for modeling yeast prion dynamics.
• To simulate the spatial and kinetic aspects of prion induction and loss in Saccharomyces cerevisiae.

Main Methods:

• Developed MIL-CELL, a multiscale computational model.
• Incorporated spatial and kinetic factors of yeast cell cycle and prion behavior.
• Utilized a relational database for data analysis and visualization.

Main Results:

• Simulated the wave-like spread of epigenetic prion elements through yeast populations.
• Demonstrated MIL-CELL's capability to model prion induction, loss, and population heterogeneity.
• Showcased the tool's utility in studying cell aging by monitoring chronological and reproductive age.

Conclusions:

• MIL-CELL provides a robust platform for investigating yeast prion phenomena.
• The model aids in understanding prion transmission dynamics and population-level effects.
• MIL-CELL is a valuable resource for research in yeast biology, epigenetics, and aging studies.