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Related Concept Videos

Reporter Genes02:11

Reporter Genes

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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Updated: Jul 17, 2025

Live-cell Imaging of Migrating Cells Expressing Fluorescently-tagged Proteins in a Three-dimensional Matrix
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Live-cell Imaging of Migrating Cells Expressing Fluorescently-tagged Proteins in a Three-dimensional Matrix

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Functional imaging-guided cell selection for evolving genetically encoded fluorescent indicators.

Chang Lin1, Lihao Liu1, Peng Zou1,2,3

  • 1College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University, Beijing 100871, China.

Cell Reports Methods
|September 6, 2023
PubMed
Summary
This summary is machine-generated.

We developed Faculae, a new imaging platform for engineering fluorescent biosensors by linking cell appearance to genetic code. This method rapidly screens thousands of variants, accelerating the creation of advanced cellular imaging tools.

Failed At:

2026-06-19T13:49:18.879588+00:00

Keywords:
directed evolutionfar-red calcium indicatorfluorescence imaginggenetically encoded fluorescent indicatorphotoactivatable cell taggingpooled screening

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