Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mismatch Repair01:20

Mismatch Repair

4.9K
Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
4.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Solid-phase synthesis and antimicrobial activities of lipid-neomycin conjugates.

Bioorganic & medicinal chemistry letters·2026
Same author

Sequence specificity of peptide nucleic acids containing terminal cationic amino acids.

Bioorganic & medicinal chemistry·2026
Same author

Hydroxy group modulated G-quadruplex binding, selectivity, and cellular uptake of styryl julolidine-benzothiazolium dyes.

Bioorganic chemistry·2026
Same author

Broad-Spectrum Activity of Peptide-Linked Amikacin Conjugates in Synergy with Polymyxin B against Extensively Drug-Resistant and Pandrug-Resistant Bacteria.

ACS infectious diseases·2025
Same author

Synergistic action between peptide-neomycin conjugates and polymyxin B against multidrug-resistant gram-negative pathogens.

Frontiers in microbiology·2025
Same author

Nucleic Acid Specificity, Cellular Localization and Reduced Toxicities of Thiazole Orange-Neomycin Conjugates.

ChemistryOpen·2024

Related Experiment Video

Updated: Jul 17, 2025

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

8.0K

Parallel G-quadruplex recognition by neomycin.

Nihar Ranjan1, Dev P Arya1

  • 1Laboratory of Medicinal Chemistry, Department of Chemistry, Clemson University, Clemson, SC, United States.

Frontiers in Chemistry
|September 6, 2023
PubMed
Summary
This summary is machine-generated.

Neomycin, an aminoglycoside, binds strongly to parallel G-quadruplex DNA structures. This discovery expands the known binding sites for aminoglycosides and could inform the design of new G-quadruplex-targeting drugs.

Keywords:
G-quadruplexITCaminoglycosideneomycinrecognition

More Related Videos

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

745
Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

1.5K

Related Experiment Videos

Last Updated: Jul 17, 2025

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

8.0K
Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

745
Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

1.5K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Drug Discovery

Background:

  • G-quadruplexes are nucleic acid structures with diverse biological roles.
  • Targeting G-quadruplexes is a promising strategy for drug development.
  • Aminoglycosides are a class of antibiotics with potential therapeutic applications.

Purpose of the Study:

  • To investigate the binding of aminoglycosides, specifically neomycin, to parallel G-quadruplex structures.
  • To characterize the binding affinity and mechanism of neomycin-G-quadruplex interactions.
  • To compare the binding of neomycin with paromomycin to G-quadruplexes.

Main Methods:

  • Calorimetric and spectroscopic studies were employed to analyze binding.
  • Circular dichroism (CD) spectroscopy was used to assess structural changes.
  • Comparative binding assays were performed with neomycin and paromomycin.

Main Results:

  • Neomycin exhibits high-affinity binding (Ka ~ 105-108 M-1) to parallel G-quadruplexes.
  • Binding affinity is influenced by G-quadruplex composition, salt concentration, and pH.
  • Neomycin binding is electrostatically driven at neutral pH and does not alter the G-quadruplex conformation.
  • Paromomycin shows significantly weaker binding compared to neomycin.

Conclusions:

  • Parallel G-quadruplexes represent high-affinity binding sites for aminoglycosides like neomycin.
  • The findings enhance understanding of aminoglycoside interactions and inform the design of novel G-quadruplex-targeting agents.
  • This research opens avenues for developing new therapeutics based on aminoglycoside-G-quadruplex interactions.