Related Experiment Video
Updated: Jul 17, 2025

Generation of Murine Monoclonal Antibodies by Hybridoma Technology
Published on: January 2, 2017
A cell hybridization-based method of generating recombinant rabbit monoclonal antibodies for detecting cytokines
Yu Peng1, Chun'e Zhang1, Minyan Deng1
1Bio-Rad (Shanghai) Life Science Research and Development Co., Ltd, Shanghai, China.
Abstract:
Recombinant rabbit monoclonal antibodies (rabbit rAbs) have shown promise in various biomedical fields. However, it is challenging and costly to generate rabbit rAbs using traditional techniques. Here we describe a convenient and cost-effective method. Using this method, we generated rabbit rAbs against mouse soluble IL-6 receptor α with affinities in the range of 10-9 to 10-12 M. The presented method is suitable for industrial and academic scientists looking to customize rabbit rAbs for their research.

