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Updated: Jul 17, 2025
Generation of Murine Monoclonal Antibodies by Hybridoma Technology
Published on: January 2, 2017
A cell hybridization-based method of generating recombinant rabbit monoclonal antibodies for detecting cytokines
Yu Peng1, Chun'e Zhang1, Minyan Deng1
1Bio-Rad (Shanghai) Life Science Research and Development Co., Ltd, Shanghai, China.
Recombinant rabbit monoclonal antibodies (rabbit rAbs) have shown promise in various biomedical fields. However, it is challenging and costly to generate rabbit rAbs using traditional techniques. Here we describe a convenient and cost-effective method. Using this method, we generated rabbit rAbs against mouse soluble IL-6 receptor α with affinities in the range of 10-9 to 10-12 M. The presented method is suitable for industrial and academic scientists looking to customize rabbit rAbs for their research.
Recombinant rabbit monoclonal antibodies (rabbit rAbs) have shown promise in various biomedical fields. However, it is challenging and costly to generate rabbit rAbs using traditional techniques. Here we describe a convenient and cost-effective method. Using this method, we generated rabbit rAbs against mouse soluble IL-6 receptor α with affinities in the range of 10-9 to 10-12 M. The presented method is suitable for industrial and academic scientists looking to customize rabbit rAbs for their research.
