New protocol to affix sample for environmental and high vacuum electron microscopy.

Area of Science:

• * Microscopy and Imaging
• * Entomology and Invertebrate Zoology
• * Materials Science

Background:

• * Electron microscopy is a vital research tool across scientific disciplines.
• * Standard methods for sample mounting and removal in electron microscopy lack detailed published procedures.
• * Existing techniques can be destructive or unsuitable for valuable specimens.

Purpose of the Study:

• * To present a rapid, cost-effective protocol for affixing and detaching samples for electron microscopy.
• * To demonstrate the applicability of this technique to diverse invertebrate taxa, including crustaceans and insects.
• * To facilitate the analysis of rare, unique, or limited-availability specimens.

Main Methods:

• * Development of a novel adhesive fluidization technique for sample manipulation.
• * Application of the method to various invertebrate specimens, including delicate insect and crustacean samples.
• * Focus on non-destructive attachment and complete removal of samples from stubs.

Main Results:

• * Successful implementation of a quick and low-cost sample fixation and removal procedure.
• * Demonstrated feasibility for analyzing individual specimens or anatomical parts.
• * Preservation of specimen integrity, allowing for recovery and repositioning after analysis.

Conclusions:

• * The developed technique offers a practical solution for electron microscopy sample handling.
• * It significantly enhances the utility of collections by enabling analysis of valuable type specimens.
• * The method is versatile and applicable to a broad range of invertebrate specimens.