Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Atomic Fluorescence Spectroscopy01:29

Atomic Fluorescence Spectroscopy

419
Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which...
419

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intracellular trafficking and nuclear compartmentalization of AAV gene delivery.

Molecular therapy. Advances·2026
Same author

Aromaticity Switching Via Rearrangement of a Pyrrolyl-Substituted Phosphenium Cation.

Inorganic chemistry·2026
Same author

AAV2 Crosslinks Actin Filaments: Implications for AAV Gene Therapy Vector Design.

bioRxiv : the preprint server for biology·2026
Same author

A dual-metal strategy for N-heterocycle coordination using nickel and aluminum.

Chemical communications (Cambridge, England)·2026
Same author

Homogeneous redox catalysed reduction of CO<sub>2</sub> by nickel cyclam catalyst and chromium-based redox mediator.

Dalton transactions (Cambridge, England : 2003)·2026
Same author

An Electron-Poor Nickel Diphosphine Complex: Synthesis, Structure, and Reactivity of Ni(dFppe)<sub>2</sub>.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Metal-Organic Framework Monoliths Derived from Emulsion-Templated Foams for Reactive Filtration.

ACS applied materials & interfaces·2026
Same journal

Binary to Quaternary Rare-Earth Phosphates: Compositional Effects on Thermal Properties and CMAS Corrosion Resistance of Environmental Barrier Coatings.

ACS applied materials & interfaces·2026
Same journal

Suture-Free Piezoelectric Band-Aid Membrane for Complex Peripheral Nerve Defects.

ACS applied materials & interfaces·2026
Same journal

Single-Precursor to Dual-Function: A Transformable Metal-Organic Framework Nanoplatform for Photocatalytic H<sub>2</sub> Evolution and CO<sub>2</sub> Reduction.

ACS applied materials & interfaces·2026
Same journal

Surfactant-Templated Synthesis of Mg-Stabilized High-Loading Co Single Atoms in Mesoporous Silica Featuring Robust Co-O Bonds for Efficient Peroxymonosulfate Activation.

ACS applied materials & interfaces·2026
Same journal

Toughening Driven by Interphase Tuning in Bioinspired Nanocomposites: From Structural Engineering to Scalable Fabrication.

ACS applied materials & interfaces·2026
See all related articles

Related Experiment Video

Updated: Jul 17, 2025

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores
09:46

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores

Published on: August 19, 2013

15.6K

An Azomethine-H-Based Fluorogenic Sensor for Formic Acid.

Mark Potter1, Suman Debnath1, Marcus W Drover1

  • 1Department of Chemistry and Biochemistry, University of Windsor, 401 Sunset Avenue, Windsor, ON N9B 3P4, Canada.

ACS Applied Materials & Interfaces
|September 6, 2023
PubMed
Summary
This summary is machine-generated.

Azomethine-H dye effectively detects formic acid (FA) vapor. This new sensor shows increased luminescence with FA, enabling sensitive detection down to 0.4 ppb, paving the way for organic acid sensing.

Keywords:
Formic acid sensorazomethine-Hfluorogenic sensorgelatin thin filmsxerogels

More Related Videos

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
08:27

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Published on: October 1, 2016

9.1K
Real Time Monitoring of Intracellular Bile Acid Dynamics Using a Genetically Encoded FRET-based Bile Acid Sensor
09:21

Real Time Monitoring of Intracellular Bile Acid Dynamics Using a Genetically Encoded FRET-based Bile Acid Sensor

Published on: January 4, 2016

10.0K

Related Experiment Videos

Last Updated: Jul 17, 2025

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores
09:46

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores

Published on: August 19, 2013

15.6K
Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
08:27

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Published on: October 1, 2016

9.1K
Real Time Monitoring of Intracellular Bile Acid Dynamics Using a Genetically Encoded FRET-based Bile Acid Sensor
09:21

Real Time Monitoring of Intracellular Bile Acid Dynamics Using a Genetically Encoded FRET-based Bile Acid Sensor

Published on: January 4, 2016

10.0K

Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Chemical Sensing

Background:

  • Formic acid (FA) is a crucial C1 feedstock and a source of hydrogen gas (H2).
  • Developing efficient FA detection methods is vital for promoting cleaner energy alternatives.
  • Azomethine-H (Az-H) dye is a commercially available compound with interesting photophysical properties.

Purpose of the Study:

  • To investigate the potential of azomethine-H (Az-H) dye for formic acid (FA) sensing.
  • To explore FA detection in both solution and solid-state formats.
  • To develop a highly sensitive fluorogenic sensor for FA vapor.

Main Methods:

  • Solution studies involving absorbance and luminescence measurements of Az-H in the presence of FA.
  • Probing Az-H/FA interactions using 1H NMR spectroscopy to understand conformational changes.
  • Incorporation of Az-H into a gelatin matrix to create a solid-state sensor for FA vapor detection.

Main Results:

  • Formic acid quenched the absorbance and luminescence of Az-H in solution, indicating an interaction.
  • FA attenuated the (E)-to-(Z) conformational change of Az-H, suggesting hydrogen bonding.
  • The solid-state Az-H/gelatin sensor exhibited a FA-dependent increase in luminescence, with a detection limit of approximately 0.4 ppb FA vapor.

Conclusions:

  • Azomethine-H dye can be utilized for sensitive detection of formic acid in both solution and solid states.
  • The observed fluorescence enhancement in the solid-state sensor is attributed to FA-induced isomerization.
  • This work presents a promising step towards practical sensors for organic acids in various chemical environments.