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Related Concept Videos

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Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material,  molecules absorb light depending on the energy required for...
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The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.
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In vivo method for evaluating sunscreen protection against high-energy visible light.

J Le Digabel1, J Filiol1, C Lauze1

  • 1R&D, Pierre Fabre Dermo-Cosmétique et Personal Care, Toulouse, France.

Journal of the European Academy of Dermatology and Venereology : JEADV
|September 6, 2023
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This study introduces a new method using multispectral imaging to assess sunscreen protection against UV and high-energy visible (HEV) light. The findings show a wide-spectrum filter offers superior protection in the HEV band.

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Area of Science:

  • Dermatology
  • Photobiology
  • Biophysics

Background:

  • Sunlight's ultraviolet (UV) radiation causes skin damage, including cancer and photoaging, through DNA damage and oxidative stress.
  • Sunscreens primarily protect by filtering UV light, measured by the Sun Protection Factor (SPF).
  • Emerging evidence indicates high-energy visible (HEV) light also contributes to biological skin damage.

Purpose of the Study:

  • To develop and validate a novel in vivo method for evaluating sunscreen efficacy across a broad spectrum of light, including UV and HEV bands.
  • To assess sunscreen protection using multispectral image analysis.
  • To provide a comprehensive evaluation of photoprotection beyond traditional SPF metrics.

Main Methods:

  • Six commercial sunscreens (five SPF 50+ with organic filters, one with phenylene bis-diphenyltriazine [TriAsorB™]) and a control were tested.
  • Multispectral images of volunteer forearms were captured before and after sunscreen application using LEDs from UV to infrared.
  • Light reflectance maps were generated to calculate UV and visible light absorbance levels.

Main Results:

  • The sunscreen with the wide-spectrum filter (TriAsorB™) demonstrated significantly higher absorbance in the HEV band (380-450 nm) compared to others.
  • All tested sunscreens exhibited similar absorbance at 365 nm (UVA).
  • Multispectral imaging effectively mapped light absorbance across different wavelengths.

Conclusions:

  • Multispectral imaging offers a simple, reliable in vivo method for assessing real-world sunscreen protection.
  • This technique evaluates protection against all photo-induced skin damage, including that from HEV radiation.
  • The study highlights the importance of considering HEV light in sunscreen efficacy evaluations.