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Glycosylated haemoglobin by affinity chromatography in diabetic and non-diabetic children

C van Heyningen, T K Hanid, I Hopkinson

    Annals of Clinical Biochemistry
    |July 1, 1986
    PubMed

    Insights

    Glycosylated hemoglobin (HbA1c) measurements using affinity chromatography were evaluated in children. Age-related reference values were established, but clinical control in diabetic children showed a weak correlation with HbA1c levels.

    Area of Science:

    • Clinical Chemistry
    • Paediatric Endocrinology

    Background:

    • Glycosylated hemoglobin (HbA1c) is a key biomarker for monitoring long-term glycemic control in diabetes.
    • Accurate HbA1c measurement is crucial for effective diabetes management, especially in paediatric populations.

    Purpose of the Study:

    • To evaluate the efficacy of affinity chromatography for measuring glycosylated hemoglobin in infants and children.
    • To establish age-related reference values for non-diabetic children.
    • To assess the correlation between HbA1c levels and clinical control in paediatric patients with insulin-dependent diabetes.

    Main Methods:

    • Affinity chromatography was employed to measure glycosylated haemoglobin.
    • Paediatric populations, including non-diabetic infants and children, were studied.
    • Correlation analysis was performed between HbA1c values and clinical assessments in diabetic children.

    Main Results:

    • Age-related reference values for glycosylated haemoglobin in non-diabetic children were determined.
    • A low correlation coefficient (r = 0.24) was observed between HbA1c measurements and the paediatrician's assessment of clinical control in 26 insulin-dependent diabetic children.

    Conclusions:

    • Affinity chromatography provides a method for glycosylated haemoglobin measurement in children.
    • Established reference values aid in interpreting results for non-diabetic paediatric populations.
    • HbA1c alone may not fully reflect clinical control in paediatric diabetes, suggesting the need for comprehensive assessment.

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