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Related Experiment Videos

Circulating and tissue-bound immune complex formation in murine malaria.

C H June, C E Contreras, L H Perrin

    Journal of Immunology (Baltimore, Md. : 1950)
    |June 1, 1979
    PubMed
    Summary

    Circulating immune complexes (CIC) form during Plasmodium berghei infection in mice, appearing after 9 days and clearing only upon cure. Their presence correlates with malarial antigens and decreased complement C3 levels.

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    Clinical immunology (Orlando, Fla.)·2010

    Area of Science:

    • Immunology
    • Parasitology
    • Pathology

    Background:

    • Plasmodium berghei infection in mice can lead to complex immunological responses.
    • Understanding immune complex formation is crucial for comprehending disease pathogenesis.

    Purpose of the Study:

    • To investigate the formation and characteristics of circulating immune complexes (CIC) during Plasmodium berghei infection.
    • To correlate CIC presence with disease progression, antibody/antigen levels, and complement C3.
    • To assess the impact of drug treatment on CIC and related markers.

    Main Methods:

    • Detection of CIC using Clq-binding and conglutinin-binding assays.
    • Analysis of CIC properties including sedimentation coefficients and DNAse resistance.
    • Assessment of glomerular and tissue-bound immunoglobulins (IgM, IgG) and complement C3 deposits.

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  • Monitoring of anti-malarial antibodies, malarial antigens, and C3 levels in serum.

    • Main Results:

    • CIC appeared on day 9, peaked on day 11, and persisted until infection clearance.
    • CIC exhibited characteristics such as 13S-21S sedimentation coefficients and DNAse resistance.
    • Glomerular IgM deposits preceded CIC, while IgG and C3 deposits were concomitant.
    • CIC presence was associated with malarial antigens, anti-malarial antibodies, and depressed C3 levels.
    • Drug treatment led to C3 normalization and clearance of CIC and antigens.

    Conclusions:

    • Circulating immune complexes are a significant feature of Plasmodium berghei infection in mice.
    • CIC formation is linked to parasite antigens, host antibodies, and complement activation.
    • Clearance of CIC and normalization of C3 levels correlate with successful parasite clearance and cure.