[A Simple and Rapid Analysis Method for Nonvolatile Amines via LC-MS/MS]
Yoshitsugu Okubo1, Tomotada Iwamoto1
1Kobe Institute of Health.
Shokuhin Eiseigaku Zasshi. Journal of the Food Hygienic Society of Japan
|September 6, 2023
Summary
A new, simple analytical method accurately measures 8 nonvolatile amines, including histamine, in various foods. This validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) method offers reliable quantification for food safety analysis.
Area of Science:
- Analytical Chemistry
- Food Science
Context:
- Accurate quantification of nonvolatile amines is crucial for food safety and quality assessment.
- Existing methods for amine analysis can be complex and time-consuming.
Purpose:
- To develop a simplified and rapid analytical method for quantifying 8 types of nonvolatile amines in diverse food matrices.
- To validate the method's accuracy and precision using real food samples.
Summary:
- A straightforward analytical procedure was established involving simple dilution and filtration of trichloroacetic acid extracts.
- Analysis was performed using liquid chromatography-tandem mass spectrometry (LC-MS/MS).
- Recovery tests across 11 food types, including seafood and processed products, demonstrated high accuracy (70–120%) and repeatability (<15% RSD) for most amines.
Impact:
- The developed method provides a validated, efficient approach for routine monitoring of nonvolatile amines in food.
- The lower limit of quantification (5–6 mg/kg) ensures sensitive detection relevant to food safety regulations.
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