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Related Concept Videos

Mass Spectrometry of Amines01:19

Mass Spectrometry of Amines

4.2K
In mass spectroscopy, amines undergo fragmentation to give parent ions with odd molecule weights. This observed mass spectrum follows the nitrogen rule: a molecule with an odd number of nitrogen atoms produces a parent ion with an odd molecular weight. The remaining fragments have an even mass.
Amines undergo fragmentation through α cleavage, producing nitrogen-containing cations—iminium ions—and alkyl radicals. Mass spectra of aromatic and cyclic aliphatic amines exhibit...
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Mass Spectrometry: Amine Fragmentation00:55

Mass Spectrometry: Amine Fragmentation

1.7K
Amines can be identified using mass spectroscopy based on their characteristic fragmentation patterns. The molecular ions of amines undergo fragmentation via ⍺-cleavage. The ⍺-cleavage of the carbon-carbon bonds in amines generates an alkyl radical and resonance-stabilized nitrogen-containing cation.
In amines, the number of nitrogen atoms affects the mass of the molecular ion, which is described by the nitrogen rule of mass spectrometry. This rule states that a compound containing...
1.7K
NMR Spectroscopy Of Amines01:19

NMR Spectroscopy Of Amines

8.9K
In proton NMR spectroscopy, primary amines and secondary amines showcase their N–H protons as a broad signal in the chemical shift range between δ 0.5 and 5 ppm. The exact position in this range depends on several factors, including sample concentration, hydrogen bonding, and the type of solvent used. Since amine protons undergo fast proton exchange in solution, the protons are labile and therefore do not participate in any splitting with adjacent protons. Thus, the observed peak is...
8.9K
Atomic Absorption Spectroscopy: Lab01:21

Atomic Absorption Spectroscopy: Lab

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For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
 Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing...
478
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

820
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
820
High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

614
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
614

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Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry
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Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry

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[A Simple and Rapid Analysis Method for Nonvolatile Amines via LC-MS/MS].

Yoshitsugu Okubo1, Tomotada Iwamoto1

  • 1Kobe Institute of Health.

Shokuhin Eiseigaku Zasshi. Journal of the Food Hygienic Society of Japan
|September 6, 2023
PubMed
Summary

A new, simple analytical method accurately measures 8 nonvolatile amines, including histamine, in various foods. This validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) method offers reliable quantification for food safety analysis.

Keywords:
LC-MS/MShistaminenonvolatile amines

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Area of Science:

  • Analytical Chemistry
  • Food Science

Context:

  • Accurate quantification of nonvolatile amines is crucial for food safety and quality assessment.
  • Existing methods for amine analysis can be complex and time-consuming.

Purpose:

  • To develop a simplified and rapid analytical method for quantifying 8 types of nonvolatile amines in diverse food matrices.
  • To validate the method's accuracy and precision using real food samples.

Summary:

  • A straightforward analytical procedure was established involving simple dilution and filtration of trichloroacetic acid extracts.
  • Analysis was performed using liquid chromatography-tandem mass spectrometry (LC-MS/MS).
  • Recovery tests across 11 food types, including seafood and processed products, demonstrated high accuracy (70–120%) and repeatability (<15% RSD) for most amines.

Impact:

  • The developed method provides a validated, efficient approach for routine monitoring of nonvolatile amines in food.
  • The lower limit of quantification (5–6 mg/kg) ensures sensitive detection relevant to food safety regulations.