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RNase L-activating 2'-5' oligoadenylates bind ABCF1, ABCF3 and Decr-1
Apurva A Govande1,2, Aleksandra W Babnis3, Christian Urban3
1Department of Microbiology, Harvard Medical School, Boston, MA 02115, USA.
The Journal of General Virology
|September 7, 2023
Summary
2-5
Area of Science:
- Innate immunity
- Molecular signaling
- Biochemistry
Background:
- Nucleotide-based second messengers like 2'-5' oligoadenylates (OAs) activate innate immune pathways.
- The antiviral role of these messengers is evolutionarily conserved across species.
Purpose of the Study:
- To identify proteins that bind 2'-5' OAs.
- To investigate the function and specificity of these interactions.
Main Methods:
- Mass spectrometry to identify OA-binding proteins.
- Biochemical characterization of protein-ligand interactions.
- Co-crystal structure analysis of mouse Decr1 and 2'-5' OA.
Main Results:
- ABCF family proteins and mouse Decr1 bind 2'-5' OA with high affinity.
- Mouse Decr1 exhibits species-specific binding.
- Structural analysis elucidated the mechanism of Decr1-OA recognition.
- No significant antiviral function or translation regulation was observed for identified proteins.
Conclusions:
- 2'-5' OA interacts with ABCF proteins and Decr1, suggesting conserved signaling roles.
- The biological significance of these interactions requires further investigation.
- 2'-5' OA may act as a signaling hub distributing signals to various proteins.
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