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A Fur-regulated type VI secretion system contributes to oxidative stress resistance and virulence in Yersinia

Yuxin Zuo1, Changfu Li1, Danyang Yu1

  • 1State Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, China.

Stress Biology
|September 7, 2023
PubMed

Insights

The ferric uptake regulator (Fur) controls Yersinia pseudotuberculosis T6SS4 expression in response to manganese levels. This manganese-responsive system enhances bacterial survival under oxidative stress and boosts virulence.

Area of Science:

  • Microbiology
  • Bacterial Pathogenesis
  • Molecular Biology

Background:

  • The type VI secretion system (T6SS) is crucial for bacterial interactions.
  • Yersinia pseudotuberculosis T6SS4's role in manganese acquisition is known, but its regulation is unclear.

Purpose of the Study:

  • To elucidate the regulatory mechanism of Yersinia pseudotuberculosis T6SS4.
  • To investigate the role of manganese ions (Mn2+) and the ferric uptake regulator (Fur) in T6SS4 regulation.

Main Methods:

  • Investigated the interaction between Fur and the T6SS4 promoter region.
  • Analyzed T6SS4 expression under varying Mn2+ concentrations and oxidative stress conditions.

Main Results:

  • Fur negatively regulates T6SS4 expression by binding to its promoter region.
  • T6SS4 is induced by low Mn2+ and oxidative stress, mediated by Fur.
  • T6SS4 facilitates Mn2+ transport for survival under oxidative stress.

Conclusions:

  • Fur acts as a Mn2+-responsive transcriptional regulator for T6SS4 in Y. pseudotuberculosis.
  • T6SS4 enhances oxidative stress resistance and virulence in Y. pseudotuberculosis.
  • This study expands understanding of T6SS regulation and function in Y. pseudotuberculosis.
Keywords:
FurManganeseOxidative stressType VI secretion systemVirulence

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