Reclamation of chromium-contaminated soil by native Cr(VI)-reducing and PHA-accumulating Bacillus aryabhattai CTSI-07

Reclamation of chromium-contaminated soil by bacteria is a big confront concerning to soil health restoration, food safety, and environmental protection. Herein, the chromium-resistant Bacillus aryabhattai CTSI-07 (MG757377) showed resistance to 1000 and 300 ppm of Cr(VI) in nutrient rich Luria Bertani (LB) and nutrient-deficient sucrose low phosphate (SLP) medium, respectively. It reduced 96.7% of Cr(VI) from contaminated soil in the presence of 100 ppm of Mg within 96 h under optimized conditions. Furthermore, Cr(VI) reduction by the bacteria was validated by Fourier transform infrared spectroscopic (FTIR) and X-ray diffraction (XRD) analysis. Besides Cr(VI) reduction, the bacterial strain also showed plant growth promoting traits like N₂ fixation and indole acetic acid (IAA) production. On the other hand, transmission electron microscopy (TEM) imaging confirmed polyhydroxyalkanoates' (PHAs) granule accumulation and 0.5 g/l of PHAs was extracted from bacterial cell using SLP medium. Infra-red (IR) spectra and proton nuclear magnetic resonance (¹H NMR) chemical shift patterns established the PHAs as polyhydroxybutyrate (PHB). Melting (Tm) and thermal degradation (Td) temperature of the PHB were 169 °C and 275 °C, respectively, as evident from thermogravimetry differential thermal analysis (TG-DTA). Atomic force microscopic (AFM) imaging depicted that the PHB film surface was rough and regular. Furthermore, the multi-metal-resistant, plant growth-promoting, and PHB-producing bacteria could reduce 99.82% of Cr(VI) from contaminated soil within 120 days in pot culture. Thus, it can be used for long-term reclamation of chromium-contaminated soil to restore soil health, provide food safety, and environmental protection.