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Updated: Jul 17, 2025

Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae
09:25

Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae

Published on: June 2, 2021

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Distinctive phosphorylation pattern during mitotic exit network (MEN) regulation is important for the development and

Wanzhen Feng1,2, Jiansheng Wang3, Xinyu Liu1,2

  • 1Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, 210095, China.

Stress Biology
|September 7, 2023
PubMed

Insights

The mitotic exit network (MEN) pathway in Magnaporthe oryzae is crucial for cell division. MoSep1 kinase phosphorylates MoMob1, essential for fungal development and pathogenicity.

Area of Science:

  • Cell Biology
  • Mycology
  • Molecular Biology

Background:

  • The mitotic exit network (MEN) pathway regulates cell division.
  • In Magnaporthe oryzae, MEN components MoSep1 and MoMob1-MoDbf2 are vital for development and pathogenicity.

Purpose of the Study:

  • To investigate the specific roles and interactions of MEN pathway components in M. oryzae.
  • To elucidate the phosphorylation mechanism of MoMob1 by MoSep1 and its functional significance.

Main Methods:

  • Gene deletion and in vitro phosphorylation assays.
  • Mass spectrometry (MS) analysis to identify phosphorylation sites.
  • Cell cycle analysis during fungal development and infection.

Main Results:

  • Deletion of MoSEP1 impacted MoMob1 phosphorylation, unlike in S. cerevisiae.
  • Serine 43 (S43) was identified as the critical phosphorylation site on MoMob1 by MoSep1.
  • MoSep1-dependent MoMob1 phosphorylation is essential for M. oryzae cell division, vegetative growth, and infection.

Conclusions:

  • MoSep1-MoMob1 interaction and phosphorylation are distinct in M. oryzae compared to yeast.
  • The MEN pathway plays conserved and unique roles in regulating M. oryzae cell cycle, development, and pathogenesis.
Keywords:
Mitotic exit network (MEN)PathogenicityPhosphorylationRice blast

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