Related Experiment Video
Updated: Jul 17, 2025
Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques
Published on: March 13, 2015
Achieving routine application of dried blood spots for erythropoietin receptor agonist analysis in doping control:
Lídia Requena-Tutusaus1,2, Indira Anselmo1, Élida Alechaga1,2
1Catalonian Antidoping Laboratory, Doping Control Research Group, Fundació IMIM (Hospital del Mar Medical Research Institute), Barcelona, Spain.
Abstract:
Background: Erythropoietin receptor agonists (ERAs) are substances prohibited in sports and currently monitored in urine and blood. There is a great interest in new matrices like dried blood spots (DBSs). Method: A direct method for the detection of ERAs in DBSs using one single spot of 25 μl has been optimized and validated. Results: Limits of detection close or equal to those required by the World Anti-Doping Agency for serum/plasma samples were achieved, using a volume 20-times lower. All analytes were stable for at least 90 days at room temperature. Conclusion: Method performance was comparable to the requirements established for blood samples and, thus, monitoring of ERAs is reliable in DBSs in the context of doping control.
