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Updated: Jul 17, 2025
Generation of Murine Monoclonal Antibodies by Hybridoma Technology
Published on: January 2, 2017
Response to Comments on "Monoclonal Antibody Discovery Based on Precise Selection of Single Transgenic Hybridomas
Yuan Li1,2, Zhanhui Wang1
1National Key Laboratory of Veterinary Public Health Security, Beijing Key Laboratory of Detection Technology for Animal Derived Food Safety, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China.
Abstract:
Listek et al. described some technical concerns for the single transgenic hybridoma selection method (STHSM) that we developed. In this work, we developed the STHSM to screen hapten-specific hybridoma cells based on a streptavidin-labeled antigen originally proposed by Listek et al. that was used for the selection of OVA-specific hybridoma. Due to the obvious differences between hapten with a single epitope and protein with multiple epitopes, we significantly innovated the specific procedures in the STHSM that could answer the questions raised by Listek's group.
