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Updated: Jun 29, 2026

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes
Published on: November 23, 2021
The TU Wien Turbulent Water Channel: Flow control loop and three-dimensional reconstruction of anisotropic particle
Vlad Giurgiu1, Giuseppe Carlo Alp Caridi1, Mobin Alipour1,2
1Institute of Fluid Mechanics and Heat Transfer, TU Wien, 1060 Wien, Austria.
Abstract:
A horizontal water channel facility was built to study particle dynamics in a turbulent flow. The channel is sufficiently long to produce fully developed turbulence at the test section, and the width-to-height ratio is sufficiently large to avoid the sidewall effect for a large proportion of the cross-section. The system was designed to study the dynamics of complex-shaped particles in wall-bounded turbulence, the characteristics of which can be finely controlled. A maximum bulk velocity of up to 0.8 m s-1 can be achieved, corresponding to a bulk Reynolds number of up to 7 × 104 (shear Reynolds number ≈1580), and flow parameters can be controlled within ±0.1%. The transparent channel design and aluminum structures allow easy optical access, which enables multiple laser and camera arrangements. With the current optical setup, a measurement volume of up to 54 × 14 × 54 mm3 can be imaged and reconstructed with six cameras from the top, bottom, and sides of the channel. Finally, the in-house developed reconstruction and tracking procedure allows us to measure the full motion of complex objects (i.e., shape reconstruction, translational, and rotational motions), and in this instance, it is applied to the case of microscopic, non-isotropic polyamide fibers.
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