A Review on Analytical Methods for Tigecycline Estimation From Its Bulk and Dosage Form

Area of Science:

• Analytical Chemistry
• Pharmaceutical Analysis

Background:

• Tigecycline (TIG), a glycylcycline antibiotic, faces stability challenges due to its complex structure, impacting its quality control.
• Its instability in solution necessitates analysis of lyophilized powder, posing challenges for accurate quantification.

Approach:

• A comprehensive literature review from 1999-2022 was conducted to identify and evaluate analytical methodologies for TIG.
• The review focuses on methods for determining TIG in bulk, lyophilized powder, and pharmacopoeial preparations.

Key Points:

• Various analytical techniques including UV-visible spectrophotometry, spectrofluorimetry, RP-HPLC, FT-IR, and electrophoresis are reported for TIG quantification and characterization.
• Reversed-phase high-performance liquid chromatography (RP-HPLC) is effective without derivatization, while fluorescence methods require it.

Conclusions:

• Existing analytical methods for tigecycline quality control vary in complexity and environmental impact.
• There is a critical need for developing simpler, faster, and more eco-friendly analytical methods for tigecycline, minimizing organic solvent use and tedious sample preparation.