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Preparation of 3D Fibrin Scaffolds for Stem Cell Culture Applications
Published on: March 2, 2012
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Stem cell culture and differentiation in 3-D scaffolds
Yasuhiko Tabata1, Idaszek Joanna2, Akon Higuchi3
1Department of Regeneration Science and Engineering, Institute for Life and Medical Sciences, Kyoto University, Kawara-cho, Shogoin, Sakyo-ku, Kyoto, Japan.
Progress in Molecular Biology and Translational Science
|September 7, 2023
Summary
Three-dimensional (3-D) cultivation methods are more efficient for preparing large numbers of human pluripotent stem (hPS) cells for clinical applications compared to traditional 2-D cultures. This chapter reviews various 3-D strategies for hPS cell culture.
Area of Science:
- Stem Cell Biology
- Biotechnology
- Tissue Engineering
Background:
- Two-dimensional (2-D) cell culture is standard for human pluripotent stem (hPS) cell analysis and signaling pathway research.
- Clinical applications require large quantities of hPS cells, which are difficult to achieve with 2-D methods.
Purpose of the Study:
- To review and compare various three-dimensional (3-D) cultivation strategies for efficient human pluripotent stem (hPS) cell expansion.
- To highlight the advantages of 3-D culture systems for clinical-scale hPS cell production.
Main Methods:
- Review of existing literature on 3-D hPS cell cultivation techniques.
- Categorization of 3-D methods including microencapsulation, microcarriers, self-aggregation, nanofibers, and macroporous scaffolds.
Main Results:
- 3-D cultivation offers superior efficiency for generating large-scale hPS cell numbers crucial for clinical treatments.
- Diverse 3-D strategies exist, each with unique advantages for cell expansion and maintenance of pluripotency.
Conclusions:
- Three-dimensional (3-D) cultivation represents a more efficient and scalable approach for human pluripotent stem (hPS) cell expansion compared to conventional 2-D methods.
- The reviewed 3-D strategies provide a foundation for optimizing hPS cell production for therapeutic applications.
