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Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing
Published on: October 3, 2018
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Biomarker Discovery by ORFeome Phage Display.
Philip Alexander Heine1, Rico Ballmann1, Praveen Thevarajah1
1Institut für Biochemie, Biotechnologie und Bioinformatik, Departments Biotechnology and Medical Biotechnology, Technische Universität Braunschweig, Braunschweig, Germany.
Methods in Molecular Biology (Clifton, N.J.)
|September 7, 2023
Summary
ORFeome phage display is a powerful technique for discovering immunogenic biomarkers. This method uses phage display to identify protein fragments from any genome, enabling biomarker discovery for various applications.
Area of Science:
- Biotechnology
- Molecular Biology
- Immunology
Background:
- Phage display is a versatile method for protein studies and antibody generation.
- It can be adapted for discovering immunogenic proteins as potential biomarkers.
Purpose of the Study:
- To introduce and detail the ORFeome phage display technique for biomarker discovery.
- To highlight its advantages over traditional methods, especially for difficult-to-cultivate organisms and metagenomic samples.
Main Methods:
- Genome or metagenome fragmentation and cloning into a phagemid vector.
- Display of protein fragments on M13 phage surface using Hyperphage (M13KO7 ΔpIII).
- Selection of immunogenic peptides/protein fragments via bio-panning using antibodies or serum samples.
Main Results:
- ORFeome phage display enables the discovery of immunogenic peptides and protein fragments.
- The method is suitable for organisms that are hard to cultivate and for metagenomic samples.
- Biomarker discovery is not limited to surface proteins, encompassing virtually all encoded peptides/fragments.
Conclusions:
- ORFeome phage display is an efficient in vitro method for biomarker discovery.
- Selected biomarkers can be utilized for developing diagnostic assays, vaccines, and in protein interaction studies.
