Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Compounds that inhibit chymotrypsin and cell replication.

B Goz, C Ganguli, M Troconis

    Biochemical Pharmacology
    |October 15, 1986
    PubMed
    Summary

    Several compounds were tested for their ability to inhibit bovine pancreatic alpha-chymotrypsin and cell replication. Inhibition constants (Ki) and half-maximal inhibitory concentrations (IC50) showed strong correlation, supporting a role for chymotrypsin-like activity in cell division.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Effect of pressure on normal and superconducting state properties of iron based superconductor PrFeAsO<sub>0.6</sub>F <sub>y</sub> (y = 0.12, 0.14).

    Scientific reports·2017
    Same author

    Extra-nuclear effects of estrogen on cortical bone in males require ERαAF-1.

    Journal of molecular endocrinology·2017
    Same author

    ESR1 and ESR2 differentially regulate daily and circadian activity rhythms in female mice.

    Endocrinology·2014
    Same author

    The effects of the phytoestrogen, coumestrol, on gonadotropin-releasing hormone (GnRH) mRNA expression in GT1-7 GnRH neurones.

    Journal of neuroendocrinology·2003
    Same author

    Structure-function relationships in estrogen receptors and the characterization of novel selective estrogen receptor modulators with unique pharmacological profiles.

    Annals of the New York Academy of Sciences·2002
    Same author

    Comparative QSAR analysis of estrogen receptor ligands.

    Chemical reviews·2001

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Enzyme Kinetics

    Background:

    • Bovine pancreatic alpha-chymotrypsin is a serine protease involved in various biological processes.
    • Cell replication is a fundamental process in all living organisms.
    • Understanding the molecular mechanisms regulating cell replication is crucial for various fields, including medicine and biotechnology.

    Purpose of the Study:

    • To investigate the relationship between the inhibition of bovine pancreatic alpha-chymotrypsin and the inhibition of cell replication.
    • To determine if a cellular chymotrypsin-like activity is essential for cell replication.

    Main Methods:

    • Enzyme inhibition assays were performed to determine the inhibition constants (Ki) for various compounds against bovine pancreatic alpha-chymotrypsin.

    Related Experiment Videos

  • Cell-based assays were conducted to measure the half-maximal inhibitory concentrations (IC50) of the same compounds on cell replication.
  • A comparative analysis of Ki and IC50 values was performed.

    • Main Results:

    • A strong correlation was observed between the Ki values for alpha-chymotrypsin inhibition and the IC50 values for cell replication inhibition across a range of compounds.
    • This agreement spanned three orders of magnitude, indicating a consistent relationship.
    • The data demonstrated that compounds inhibiting alpha-chymotrypsin also effectively inhibited cell replication.

    Conclusions:

    • The findings strongly support the hypothesis that a cellular, chymotrypsin-like enzymatic activity is necessary for cell replication.
    • This suggests that targeting chymotrypsin-like enzymes could be a viable strategy for controlling cell proliferation.
    • Further research into specific cellular chymotrypsin-like proteases involved in cell division is warranted.