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Updated: Jul 17, 2025
Isolation and Culture of Primary Human Gingival Epithelial Cells using Y-27632
Published on: November 6, 2021
Effect of polydeoxyribonucleotide with xenogeneic collagen matrix on gingival phenotype modification: a pilot
Hyun-Chang Lim1, Chang-Hoon Kim2, Han-Kyu Lee2
1Department of Periodontology, College of Dentistry, Kyung Hee University, Periodontal-Implant Clinical Research Institute, Kyung Hee University Medical Center, Seoul, Korea. Hyun-Chang.Lim@khu.ac.kr.
Purpose:
To investigate the effect of xenogeneic collagen matrix (XCM) with polydeoxyribonucleotide (PDRN) for gingival phenotype modification compared to autogenous connective tissue graft.
Methods:
Five mongrel dogs were used in this study. Box-type gingival defects were surgically created bilaterally on the maxillary canines 8 weeks before gingival augmentation. A coronally positioned flap was performed with either a subepithelial connective tissue graft (SCTG) or XCM with PDRN (2.0 mg/mL). The animals were sacrificed after 12 weeks. Intraoral scanning was performed for soft tissue analysis, and histologic and histomorphometric analyses were performed.
Results:
One animal exhibited wound dehiscence, leaving 4 for analysis. Superimposition of STL files revealed no significant difference in the amount of gingival thickness increase (ranging from 0.69±0.25 mm to 0.80±0.31 mm in group SCTG and from 0.48±0.25 mm to 0.85±0.44 mm in group PDRN; P>0.05). Histomorphometric analysis showed no significant differences between the groups in supracrestal gingival tissue height, keratinized tissue height, tissue thickness, and rete peg density (P>0.05).
Conclusions:
XCM soaked with PDRN yielded comparable gingival augmentation to SCTG.
