The novel HLA-A*02:405 allele characterized by sequencing-based typing
Xiao-Feng Li1,2,3,4, Xu Zhang1,2,3, Feng-Qiu Lin1,2,3
1Liaoning Blood Center, Transfusion Medicine Institute, Shenyang, China.
HLA
|September 8, 2023
Summary
The human leukocyte antigen (HLA) A*02:405 allele is distinguished from HLA-A*02:06:01:01 by a single nucleotide substitution. This genetic variation occurs in codon 161 within exon 3.
Area of Science:
- Immunogenetics
- Molecular biology
- Human leukocyte antigen (HLA) system
Background:
- The HLA system plays a critical role in immune response and transplantation.
- Accurate HLA allele identification is essential for clinical applications and immunological studies.
- Allelic variations within HLA genes can impact immune recognition and disease susceptibility.
Purpose of the Study:
- To characterize a novel HLA-A allele, HLA-A*02:405.
- To identify the specific genetic differences between HLA-A*02:405 and a closely related allele, HLA-A*02:06:01:01.
Main Methods:
- Nucleotide sequencing of relevant HLA gene regions.
- Comparative sequence analysis to identify genetic variations.
- Bioinformatic tools for HLA allele nomenclature and characterization.
Main Results:
- The HLA-A*02:405 allele differs from HLA-A*02:06:01:01 by a single nucleotide substitution.
- This substitution is located at codon 161 in exon 3 of the HLA-A gene.
Conclusions:
- The identified nucleotide difference defines HLA-A*02:405 as a distinct allele.
- This finding contributes to the growing database of HLA polymorphism.
- Understanding such variations is crucial for high-resolution HLA typing and immunological research.
Related Concept Videos
01:49Multiple Allele Traits
The Concept of Multiple Allelism
01:11Genome-wide Association Studies-GWAS
Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...
GWAS does not require the identification of the target gene involved in...
01:29Modern Molecular Taxonomy
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...