Comprehensive tool for a phase compensation reconstruction method in digital holographic microscopy operating in...

Area of Science:

• Optics and Photonics
• Computational Imaging
• Microscopy

Background:

• Digital Holographic Microscopy (DHM) is crucial for quantitative phase imaging (QPI) in materials and biology.
• Accurate phase measurements in DHM depend heavily on computational reconstruction algorithms.
• Non-telecentric DHM systems are common but introduce spherical wavefront distortions requiring compensation.

Purpose of the Study:

• To develop a simplified and robust computational reconstruction method for non-telecentric DHM.
• To reduce the number of required parameters for accurate phase compensation.
• To provide an accessible tool for undistorted phase imaging.

Main Methods:

• Reformulated reconstruction approach based on Kemper's group work.
• Algorithm involves six main steps: diffraction order selection, wavefront curvature estimation, spatial filtering, interference angle compensation, wavefront center estimation, and optional optimization.
• Reduced required parameters to sensor pixel size and source wavelength.

Main Results:

• Successfully compensated spherical wavefront distortions in non-telecentric DHM.
• Achieved accurate and undistorted phase measurements.
• Developed an algorithm with minimal user input, requiring only pixel size and wavelength.

Conclusions:

• The proposed method offers a simplified yet effective approach for phase reconstruction in non-telecentric DHM systems.
• Open-access codes and a user interface tool are provided for practical implementation.
• This work facilitates more accessible and accurate quantitative phase imaging.