A simple synthesis of a core-shell structure PPy-Au nanocomposite for immunosensing of C-reactive protein

High-sensitivity C-reactive protein (hs-CRP) is an inflammatory biomarker and can accurately predict the development of cardiovascular disease (CVD). We synthesized a core-shell structure PPy-Au nanocomposite in situ by chemically oxidizing pyrrole (Py) with HAuCl₄ and the produced Au nanoparticles realized the doping in the polymerization. Analysis of morphology and energy spectrum as well as electrochemical characterization confirmed the successful one-pot synthesis. The conductive polymers with porous structure provide abundant sites for anti-CRP binding and effectively enhanced the sensitivity of the label-free BSA/anti-CRP/PPy-Au/GCE immunosensor. Its analytical performance was observed using differential pulse voltammetry (DPV), with a linear range from 0.0005 to 60 μg mL^-1 and a detection limit of 0.17 ng mL^-1. The platform demonstrated satisfactory selectivity, stability, and reproducibility. To validate its clinical application, we detected CRP in human serum samples with a recovery rate of 101.00-105.95% and investigated the consistency of the developed method and immunoturbidimetry with a deviation between -1.2% and +3.2%, suggesting great potential for use in point-of-care testing (POCT).