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Updated: Jul 16, 2025
ELIME Enzyme Linked Immuno Magnetic Electrochemical Method for Mycotoxin Detection
Published on: October 23, 2009
Magnetic-fluorescent immunosensing platform applying AuNPs heterogeneous MIL-53(Al) composite for efficient detection
Huilin Li1, Jingying Yang1, Ran Han1
1State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, China; Key Laboratory of Food Quality and Health of Tianjin, Tianjin University of Science & Technology, 300457 Tianjin, China.
Abstract:
Rapid, sensitive, specific and stable detection of mycotoxin in food remains an extremely crucial issue. Herein, a magnetic-fluorescent immunosensing platform for the detection of zearalenone (ZEN) was proposed. The platform utilized Au nanoparticles (AuNPs) heterogeneous fluorescent metal-organic framework (MIL-53(Al)@AuNPs) labeled with ZEN-bovine serum albumin (ZEN-BSA) as signal probe and ZEN mono-antibodies coupled with magnetic NPs (MNPs-mAbs) as capture probe. Specifically, the heterogenization of AuNPs on the MIL-53(Al) surface improved its biocompatibility, and facilitated the loading of ZEN-BSA conjugates. The MNPs-mAbs could rapidly capture the target ZEN, simplify the immunoassay process and further improve the detection efficiency. The established competitive magnetic-fluorescent immunosensing platform had a wider linear response to ZEN in the range of 0.001-100 ng/mL with a lower limit of detection (LOD) at 0.0035 ng/mL, and could finish the whole detection process within 20 min, showing great potential for rapid and sensitive detection of food contaminants.
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