Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Meet the author: Kavitha Sarma.

Kavitha Sarma

    Molecular Cell
    |September 8, 2023
    PubMed
    Summary
    This summary is machine-generated.

    G-quadruplexes and R-loops cooperate to enhance CTCF binding, a crucial step in genome organization. This finding offers new insights into the regulation of DNA architecture and gene expression.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Human macrophages encode stimulus-specific information of prior exposures through trained immunity.

    Cell systems·2026
    Same author

    Nuclear export of R-loop by the DDX1 and XPO1 complex promotes senescence-associated secretory phenotype and inflammaging.

    Nature aging·2026
    Same author

    ADNP regulates chromatin architecture and lineage fidelity during neural differentiation.

    PLoS genetics·2026
    Same author

    AP-1 mediates cellular adaptation and memory formation.

    Nature communications·2026
    Same author

    Gastruloid patterning reflects division of labor among biased stem cell clones.

    bioRxiv : the preprint server for biology·2025
    Same author

    The paradox of R-loops: guardians of the genome or drivers of disease?

    Genome research·2025
    Same journal

    Thyroid cancer-associated EZH1 Q571R mutation drives chromatin compaction and H3K27me3 invasion into active chromatin.

    Molecular cell·2026
    Same journal

    Genome-wide rotational and translational phasing of nucleosomes with human transcription factors.

    Molecular cell·2026
    Same journal

    Spliceosomal proofreading factors safeguard 3' splice-site fidelity and prevent proteotoxicity and inflammation.

    Molecular cell·2026
    Same journal

    Cytosolic EZH2-IMPDH2 complexes regulate melanoma progression and metastasis via GTP.

    Molecular cell·2026
    Same journal

    A bacterial reverse transcriptase: Protein-templated DNA synthesis fuels antiviral immunity.

    Molecular cell·2026
    Same journal

    Tweezing apart ribosome heterogeneity.

    Molecular cell·2026
    See all related articles

    Area of Science:

    • Genomics
    • Molecular Biology
    • Epigenetics

    Background:

    • G-quadruplexes are non-canonical DNA structures.
    • R-loops are DNA:RNA hybrids.
    • CTCF is a key architectural protein in the genome.

    Purpose of the Study:

    • To investigate the interplay between G-quadruplexes, R-loops, and CTCF binding.
    • To understand how these structures influence genome organization.

    Main Methods:

    • Chromatin immunoprecipitation sequencing (ChIP-seq)
    • DNA:RNA immunoprecipitation (DRIP)
    • Analysis of G-quadruplex forming sequences

    Main Results:

    • G-quadruplexes and R-loops co-localize at specific genomic regions.

    Related Experiment Videos

  • The presence of both G-quadruplexes and R-loops enhances CTCF binding affinity.
  • This cooperative binding influences chromatin looping and gene regulation.

    • Conclusions:

    • G-quadruplexes and R-loops act synergistically to promote CTCF binding.
    • This mechanism provides a novel layer of regulation for genome architecture.
    • Understanding this interplay is vital for comprehending gene expression control.