The effect of high-fat diets on microsomal lauric acid hydroxylation in rat liver

A diet with 20% (w/w) fish oil or partially hydrogenated fish oil has been shown to stimulate omega-oxidation of lauric acid 2.5-fold with rat liver microsomal preparations after 1 week of feeding. A diet containing either 20% (w/w) soybean oil, partially hydrogenated soybean oil or rapeseed oil had no effect. The omega-oxidation was also stimulated by fasting (3.7-fold) and by clofibrate (13-fold). The stimulation of omega-oxidation with partially hydrogenated fish oil was at its highest level after 3 days of feeding, and was dose dependent in the dietary oil of range 5-25% (w/w). With various high-fat diets, a high correlation was found (r = 0.81) between peroxisomal beta-oxidation of palmitoyl-CoA and microsomal omega-oxidation of lauric acid.