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A comprehensive genomic catalog from global cold seeps.

Yingchun Han1, Chuwen Zhang1, Zhuoming Zhao1

  • 1Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, 361005, China.

Scientific Data
|September 8, 2023
PubMed
Summary
This summary is machine-generated.

Global cold seeps host diverse microbes crucial for biogeochemical cycles. This study catalogs microbial genes and genomes, revealing novel species and expanding our understanding of these unique ecosystems.

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Area of Science:

  • Microbiology
  • Genomics
  • Biogeochemistry

Background:

  • Cold seeps are vital microbial ecosystems influencing global biogeochemical cycles.
  • Existing metagenomic studies offer insights but lack comprehensive global diversity and genetic data.

Purpose of the Study:

  • To construct a comprehensive gene and genome catalog of global cold seep microbes.
  • To identify novel microbial species and their genetic repertoire in cold seeps.

Main Methods:

  • Compiled 165 metagenomic datasets from 16 global cold seep sites.
  • Constructed a non-redundant gene catalog and generated metagenome-assembled genomes (MAGs).

Main Results:

  • Created a gene catalog of 147 million genes, with 36% unassigned functions.
  • Obtained 3,164 species-level MAGs, with 94% representing novel species.
  • Identified 81 archaeal anaerobic methanotrophic (ANME) and 23 syntrophic sulfate-reducing bacteria (SRB) species.

Conclusions:

  • The developed catalog provides a valuable resource for studying cold seep microbiome functions.
  • This work significantly expands the known diversity and genetic potential of cold seep microbial communities.