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High Thioredoxin Domain-Containing Protein 11 Expression Is Associated with Tumour Progression in Glioma
Ying-Tso Chen1,2, Chia-Li Chung3, Yu-Wen Cheng1,2
1Division of Neurosurgery, Department of Surgery, Kaohsiung Veterans General Hospital, Kaohsiung 813414, Taiwan.
International Journal of Molecular Sciences
|September 9, 2023
Summary
Thioredoxin domain-containing protein 11 (TXNDC11) is linked to poor prognosis in glioblastoma (GBM). Silencing TXNDC11 inhibits GBM cell growth and invasion, suggesting it as a potential therapeutic target for brain tumors.
Area of Science:
- Neuro-oncology
- Molecular Biology
- Cancer Research
Background:
- Glioblastoma (GBM) is an aggressive brain tumor with poor patient outcomes despite standard treatments.
- Thioredoxin domain-containing protein 11 (TXNDC11) is an endoplasmic reticulum stress-induced protein with an unclear role in GBM.
Purpose of the Study:
- To investigate the prognostic significance of TXNDC11 in glioblastoma.
- To explore the functional role of TXNDC11 in GBM cell proliferation, invasion, migration, apoptosis, and temozolomide sensitivity.
Main Methods:
- Clinical data and TXNDC11 expression levels were analyzed in glioma patients.
- In vitro assays (proliferation, invasion, migration, apoptosis, temozolomide sensitivity) were performed using GBM cells with TXNDC11 manipulation (siRNA silencing and overexpression).
- Western blotting was used to assess protein expression (N-cadherin, E-cadherin, Cyclin D1). In vivo orthotopic xenotransplantation models were utilized.
Main Results:
- High TXNDC11 expression correlated significantly with higher World Health Organization (WHO) grade and poorer prognosis in gliomas.
- TXNDC11 silencing inhibited GBM cell proliferation, migration, and invasion, while promoting apoptosis.
- TXNDC11 knockdown altered the expression of key proteins involved in cell adhesion and proliferation (N-cadherin, E-cadherin, Cyclin D1).
- In vivo studies showed that TXNDC11-silenced GBM cells exhibited slower tumor growth and prolonged survival in nude rats.
Conclusions:
- TXNDC11 is an independent prognostic factor for glioma and acts as a potential oncogene in GBM.
- TXNDC11 represents a promising therapeutic target for glioblastoma treatment.
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