Highly Efficient Fluorescent Detection of Vitamin B₁₂ Based on the Inner Filter Effect of Dithiol-Functionalized...

We report a fluorescent assay for the determination of vitamin B₁₂ (VB₁₂) based on the inner filter effect (IFE) of 1,3-propanedithiol-functionalized silver nanoparticles (PDT-AgNPs). PDT was simply functionalized on the surface of AgNPs through Ag-thiol interaction, which leads to significantly enhanced fluorescence, with excitation and emission at 360 and 410 nm, respectively, via their thiol-mediated aggregation. Since target VB₁₂ has strong absorption centered at 360 nm, which is almost completely overlapping with the excitation spectra of PDT-AgNPs, the VB₁₂ induced strong quenching of the fluorescence of PDT-AgNPs via IFE. The IFE-based mechanism for the fluorescence quenching of PDT-AgNPs in the presence of VB₁₂ was confirmed by the analyses of Stern-Volmer plots at different temperatures and fluorescence decay curves. The fluorescence-quenching efficiency of PDT-AgNPs was linearly proportional to the concentration of VB₁₂ in a wide range of 1 to 50 μM, with a lower detection limit of 0.5 μM, while preserving excellent selectivity toward target VB₁₂ among possible interfering molecules. Furthermore, the PDT-AgNPs-mediated assay succeeded in quantitatively detecting VB₁₂ in drug tablets, indicating that PDT-AgNPs can serve as an IFE-based fluorescent probe in pharmaceutical preparations by taking advantages of its ease of use, rapidity, and affordability.