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Next-generation Sequencing03:00

Next-generation Sequencing

89.9K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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Sanger Sequencing01:57

Sanger Sequencing

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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Related Experiment Video

Updated: Jul 16, 2025

DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications
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DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications

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Recent Advances in DNA Nanomaterials.

Incherah Bekkouche1, Maria N Kuznetsova1, Dovlet T Rejepov1

  • 1Nanotechnology Scientific and Educational Center, Institute of Biochemical Technology and Nanotechnology, Peoples' Friendship University of Russia n.a. P. Lumumba (RUDN), Miklukho-Maklaya St. 6, Moscow 117198, Russia.

Nanomaterials (Basel, Switzerland)
|September 9, 2023
PubMed
Summary

DNA-containing nanomaterials (DNA-NMs) offer unique properties for medicine and engineering. This review highlights advancements in DNA-NMs while addressing challenges in precise control and targeted delivery for nanomanipulation applications.

Keywords:
DNAaptamersmicrocirclesnanomaterialsplasmid

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Area of Science:

  • Nanotechnology
  • Biomaterials Science
  • Molecular Engineering

Background:

  • DNA-containing nanomaterials (DNA-NMs) are gaining prominence due to their small size, controllability, clustering, and permeability.
  • These properties make DNA-NMs valuable in medicine, environment, and engineering.

Purpose of the Study:

  • To review recent advancements in DNA-NMs.
  • To explore the integration of DNA-NMs across various scientific disciplines.
  • To identify persistent challenges and future research directions in nanomanipulation.

Main Methods:

  • Literature review of recent progress in DNA-NMs.
  • Analysis of DNA-NM applications in diverse fields.
  • Discussion of current limitations and future prospects.

Main Results:

  • DNA-NMs demonstrate significant potential in various scientific and industrial applications.
  • Integration of DNA-NMs is observed across in vivo/in vitro studies, microcircle excisions, and plasmid oligomers.
  • Challenges remain in precise cluster control, in vivo targeted drug distribution, and cellular micro-nano operations.

Conclusions:

  • DNA-NMs are a rapidly advancing field with broad applicability.
  • Further research is needed to overcome current limitations in control and delivery.
  • This review aims to stimulate new research perspectives and advance nanomanipulation.