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Related Experiment Video

Updated: Jul 16, 2025

Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes
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Identification and Localization of Cell Types in the Mouse Olfactory Bulb Using Slide-SeqV2.

Ai Fang1, Kaitlyn Petentler1, Andrew Price1

  • 1Stowers Institute for Medical Research, Kansas City, MO, USA.

Methods in Molecular Biology (Clifton, N.J.)
|September 9, 2023
PubMed
Summary
This summary is machine-generated.

Slide-SeqV2 visualizes gene expression in the mouse olfactory bulb by mapping RNA molecules to their locations. This spatial transcriptomics method identifies distinct cell types and subtypes within the olfactory bulb.

Keywords:
GABAergic interneuronsMitral and tufted cellsMouseNeural developmentOlfactory bulbSlide-SeqV2Spatial transcriptome

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Area of Science:

  • Neuroscience
  • Genomics
  • Molecular Biology

Background:

  • Spatial transcriptomics provides insights into tissue architecture by mapping gene expression.
  • The mouse olfactory bulb (OB) is a complex brain region crucial for processing olfactory information.

Purpose of the Study:

  • To apply Slide-SeqV2 for high-resolution spatial gene expression mapping in the mouse olfactory bulb.
  • To identify and localize diverse cell types and subtypes within the OB using spatial transcriptomics data.

Main Methods:

  • Utilized Slide-SeqV2, a spatial transcriptomics technology, to capture and spatially resolve individual RNA molecules.
  • Employed spatially barcoded beads to link transcriptomic data with precise tissue locations.
  • Analyzed gene expression profiles to identify and map cell populations within the OB.

Main Results:

  • Successfully visualized gene expression patterns across the mouse olfactory bulb.
  • Identified distinct spatial expression profiles corresponding to various known and novel cell types and subtypes.
  • Demonstrated the ability of Slide-SeqV2 to unbiasedly map cell populations based on their unique transcriptomic signatures.

Conclusions:

  • Slide-SeqV2 is a powerful tool for high-resolution spatial transcriptomics in complex brain tissues like the OB.
  • The study reveals the intricate spatial organization of cell types within the mouse olfactory bulb.
  • This approach facilitates a deeper understanding of tissue architecture and cellular heterogeneity in neuroscience research.