A new lysosome-targeted Cys probe and its application in biology and food samples

Cysteine (Cys) is a sulfur-containing amino acid that plays an important role in living systems. The most common way to supplement the body with exogenous Cys is through the consumption of Cys-rich foods. Therefore, it is important to detect and analyze Cys in living systems and food samples. However, most of the Cys fluorescent probes developed so far are limited to the detection of the cellular environment only, and very few probes can take into account the detection of Cys in plant roots and food samples. In this paper, a novel fluorescent probe LN-NCS targeting the detection of Cys in lysosomes was designed and synthesized by modifying the naphthalimide fluorophore. The probe LN-NCS has a large Stokes shift (140 nm), low cytotoxicity, low detection limit (16.3 nM), and high selectivity, and probe LN-NCS reacts with Cys to produce the compound LN-NH₂ with good fluorescence quantum yield (Ф = 0.81). Probe LN-NCS can be used to detect Cys in cells, zebrafish, plant roots, food samples, and environmental water samples. In addition, by modeling cellular inflammation, we have demonstrated that probe LN-NCS can detect changes in Cys concentration induced by cellular inflammation, providing a potential tool to better study the cellular inflammatory environment.