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Updated: Jul 16, 2025

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores
Published on: August 19, 2013
Sensitive fluorescent detection of o-aminophenol by hemicyanine boronic acid
Zhenming Hao1, Liming Hu1, Ruyu Yan1
1Faculty of Environment and Life, Beijing Key Laboratory of Environmental and Viral Oncology, Beijing University of Technology, Beijing 100124, China.
Abstract:
O-Aminophenol (OAP) is widely used in various industries, but it can have severe negative impacts on both the environment and human health. Herein, we reported the development of hemicyanine boronic acid (HBA) for the fluorescent detection of OAP. The reaction of HBA with OAP produced a strong fluorescence emission at 675 nm because of the generation of tricyclic borate ester hemicyanine (TBEH). The detection was very rapid, sensitive and specific. The detection had a linear range 0.1 - 10.0 µM in ethanol with a detection limit of 60 nM in water and ethanol. The accuracy and precision of our results were successfully verified via HPLC analysis. Our study offers a valuable tool for the facile and efficient detection of OAP, with practical applications in environmental and health management.
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