Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Trends in anxiety and depressive symptoms among adults in Norway based on eight population-based surveys from 1995 to 2024.

Discover mental health·2026
Same author

Beyond the two-conformer model: boat conformers provide stereoselectivity in S<sub>N</sub>1-type glycosylations of <i>manno</i>-type donors.

Chemical science·2026
Same author

Oseltamivir aziridines are potent influenza neuraminidase inhibitors and imaging agents.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Calpain small subunit homodimerization is robust and calcium-independent.

FEBS letters·2026
Same author

Fertility classification of the cow and peri-estrus sex-steroid hormones is associated with endometrial epithelial cell responses to conceptus and inflammatory signals.

Cell and tissue research·2026
Same author

Hypervalent chalcogenonium organocatalysis for the direct stereoselective synthesis of deoxyglycosides from hemiacetals.

Chemical science·2026

Related Experiment Video

Updated: Jul 16, 2025

LabVIEW-operated Novel Nanoliter Osmometer for Ice Binding Protein Investigations
09:32

LabVIEW-operated Novel Nanoliter Osmometer for Ice Binding Protein Investigations

Published on: February 4, 2013

20.7K

Droplet freezing assays using a nanoliter osmometer.

Jocelyn C Lee1, Thomas Hansen1, Peter L Davies1

  • 1Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, K7L 3N6, Canada.

Cryobiology
|September 9, 2023
PubMed
Summary

Accurately measuring ice nucleation temperatures is crucial for studying biological ice nucleators. A commercial nanoliter osmometer was reproducibly used to measure ice nucleation temperatures near homogeneous nucleation.

More Related Videos

Rapid Freezing using Sandwich Freezing Device for Good Ultrastructural Preservation of Biological Specimens in Electron Microscopy
09:03

Rapid Freezing using Sandwich Freezing Device for Good Ultrastructural Preservation of Biological Specimens in Electron Microscopy

Published on: July 19, 2021

5.4K
Particle Templated Emulsification enables Microfluidic-Free Droplet Assays
11:03

Particle Templated Emulsification enables Microfluidic-Free Droplet Assays

Published on: March 9, 2021

5.8K

Related Experiment Videos

Last Updated: Jul 16, 2025

LabVIEW-operated Novel Nanoliter Osmometer for Ice Binding Protein Investigations
09:32

LabVIEW-operated Novel Nanoliter Osmometer for Ice Binding Protein Investigations

Published on: February 4, 2013

20.7K
Rapid Freezing using Sandwich Freezing Device for Good Ultrastructural Preservation of Biological Specimens in Electron Microscopy
09:03

Rapid Freezing using Sandwich Freezing Device for Good Ultrastructural Preservation of Biological Specimens in Electron Microscopy

Published on: July 19, 2021

5.4K
Particle Templated Emulsification enables Microfluidic-Free Droplet Assays
11:03

Particle Templated Emulsification enables Microfluidic-Free Droplet Assays

Published on: March 9, 2021

5.8K

Area of Science:

  • Cryobiology
  • Biophysics
  • Materials Science

Background:

  • Accurate ice nucleation temperature measurement is vital for understanding biological ice nucleators.
  • Existing custom-built instruments are not commercially available.
  • Antifreeze proteins' thermal hysteresis activity requires precise nucleation temperature assays.

Purpose of the Study:

  • To reproducibly measure ice nucleation temperatures using a commercially available instrument.
  • To demonstrate the utility of a nanoliter osmometer for ice nucleation studies.
  • To achieve measurements close to the homogeneous nucleation temperature (-38 °C).

Main Methods:

  • Utilized a commercially available nanoliter osmometer for ice nucleation temperature measurements.
  • Employed an oil immersion format on 12-well grids to prevent droplet evaporation and surface nucleation.
  • Leveraged the instrument's wide operating temperature range and fine temperature control.

Main Results:

  • Successfully measured ice nucleation temperatures reproducibly, approaching the homogeneous nucleation limit of -38 °C.
  • Demonstrated the nanoliter osmometer's capability for precise thermal hysteresis activity assays of antifreeze proteins.
  • Obtained results consistent with those from other established ice nucleation measurement instruments.

Conclusions:

  • A commercially available nanoliter osmometer can be effectively used to measure ice nucleation temperatures with high reproducibility.
  • This method provides a viable alternative to custom-built instruments for studying biological ice nucleators.
  • The instrument's features facilitate accurate measurements, crucial for cryobiology and antifreeze protein research.