Amorphous copper(II)-cyanoimidazole frameworks as peroxidase mimics for hydrogen sulfide assay

Metal-organic frameworks with hierarchical porosities and exposed active sites are promising for ideal enzyme mimics. In this work, we developed a simple and feasible air oxidation strategy to prepare amorphous Cu(II)-cyanoimidazole frameworks (aCu(II)-CIFs) using CuI as the metal source in dimethylsulfoxide. Benefiting from coordination unsaturation and hierarchical porosities, aCu(II)-CIFs exhibit inherent peroxidase-mimic activity for rapid colorimetric reaction of 3,3',5,5'-tetramethylbenzidine (TMB). aCu(II)-CIFs were utilized to develop a colorimetric platform for specific H₂S assay in the range of 0.6-30 μM, achieving a limit of detection (LOD) of 0.071 μM. Structural collapse of aCu(II)-CIFs and subsequent generation of stable CuS particles, along with reducibility of H₂S, are likely responsible for suppressing TMB_ox conversion. The proposed method successfully detected H₂S in real water samples, with a relative standard deviation (RSD) lower than 8.4%. This contribution is expected to offer unique insights into the amorphization mechanisms of MOFs and their potential applications.