Assessing fresh water acute toxicity with Surface-Enhanced Raman Scattering (SERS)

It's well known that the toxicity of chemicals in the environment depends not only their concentrations, but more importantly, their bio-availability. Thus, the acute toxicity test of environmental water samples is of great importance in water quality evaluation. In this work, water acute toxicity was determined via SERS approach for the first time based on the reaction between Escherichia coli (E. coli) and p-benzoquinone (BQ). The E. coli was used as the subject of toxicity assay. Under normal conditions, the BQ molecules can be transformed into Hydroquinone (HQ) by the E. coli bacteria; subsequently, the BQ will continue to react with the resulting HQ to form Quinone hydroquinone (QHQ). This process could be impaired in the presence of many toxic chemicals. Bromide modified Ag NPs was then introduced for the highly sensitive SERS detection of the product (HQ and QHQ). Several key factors that may affect water acute toxicity evaluation have been explored, which include the initial BQ and E. coli concentration, the incubation time with BQ, and the sodium chloride concentration. Later, the established system was applied for the toxicity evaluation of Cu²⁺. It was found that the IC₅₀ value of Cu²⁺ was 0.94 mg/L, which is superior compared with literature report. This study provides a promising SERS method for assessing acute toxicity in water bodies with high sensitivity and short detection time.