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Related Experiment Video

Updated: Jul 16, 2025

Delivery of the Cas9/sgRNA Ribonucleoprotein Complex in Immortalized and Primary Cells via Virus-like Particles "Nanoblades"

Delivery of the Cas9/sgRNA Ribonucleoprotein Complex in Immortalized and Primary Cells via Virus-like Particles "Nanoblades"

Published on: March 31, 2021

A catch-and-release nano-based gene delivery system.

Christoph O Franck¹, Andrea Bistrovic Popov¹, Ishtiaq Ahmed¹

¹BioNano Engineering Lab, Department of Chemical Engineering and Biotechnology, University of Cambridge, Philippa Fawcett Drive, Cambridge CB3 0AS, UK. lf389@cam.ac.uk.

Nanoscale Horizons

|September 11, 2023

Summary

Researchers developed a novel catch-and-release gene delivery system using modified nanoparticles. This system efficiently delivers plasmid DNA (pDNA) to human cells, showing promise for clinical gene therapy applications.

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Area of Science:

Biomaterials Science
Nanotechnology
Gene Therapy

Background:

Developing effective and safe gene delivery systems is crucial for clinical applications.
Biopolymers offer advantageous properties like biodegradability and biocompatibility for gene carriers.
Polydopamine nanoparticles are explored for their potential in drug and gene delivery.

Purpose of the Study:

To create a novel catch-and-release gene delivery system using modified polydopamine nanoparticles.
To evaluate the synergistic effects of poly-L-arginine and poly-L-histidine in pDNA delivery.
To assess the efficiency of the system in terms of pDNA binding, release, and transfection.

Main Methods:

Modification of melanin-like polydopamine nanoparticles with poly-L-arginine and poly-L-histidine blends.
Evaluation of pDNA binding affinity, cell viability, gene release, and transfection efficacy.
Comparison of the novel system's performance with a commercial transfection agent (Lipofectamine).

Main Results:

Poly-L-arginine functionalization was essential for pDNA binding but hindered intracellular release.
Co-formulation with poly-L-histidine enabled optimal pDNA release and enhanced protein expression.
The developed catch-and-release system demonstrated transfection efficacy comparable to Lipofectamine.

Conclusions:

A synergistic catch-and-release mechanism using poly-L-arginine and poly-L-histidine on polydopamine nanoparticles facilitates efficient gene delivery.
This strategy offers a promising approach for developing clinically applicable gene delivery systems.
The catch-and-release surface modification can be applied to other nanocarriers and surfaces.