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Updated: Jul 16, 2025

Chromatin Immunoprecipitation ChIP of Histone Modifications from Saccharomyces cerevisiae
Published on: December 29, 2017
The Pho23-Rpd3 histone deacetylase complex regulates the yeast metabolic transcription factor Stb5
Elizabeth Delorme-Axford1, Tabassum Ahmad Tasmi1, Daniel J Klionsky2
1Biological Sciences, Oakland University, Rochester, Michigan, United States.
Abstract:
Macroautophagy/autophagy is an essential catabolic process for maintaining homeostasis and cell survival under stressful conditions. We previously characterized the metabolic transcription factor Stb5 as a negative modulator of autophagy through its regulation of genes involved in NADPH production. However, the molecular mechanisms regulating STB5 expression are not fully characterized. Here, we identify the yeast Pho23-Rpd3 histone deacetylase complex as a transcriptional regulator of STB5 . Our work provides insight into the mechanisms modulating the metabolic transcription factor Stb5 and expands on the repertoire of genes targeted by the Pho23-Rpd3 complex.
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