CircMAPK9 promotes adipogenesis through modulating hsa-miR-1322/FTO axis in obesity

Area of Science:

• Biochemistry
• Molecular Biology
• Genetics

Background:

• Circular RNAs (circRNAs) are emerging as key regulators in lipid metabolism.
• The precise mechanisms by which circRNAs influence obesity remain largely unknown.

Purpose of the Study:

• To elucidate the role and regulatory mechanism of circRNAs in obesity.
• To investigate the function of circMAPK9 in adipogenesis.

Main Methods:

• Whole transcriptome sequencing identified differentially expressed circRNAs.
• RT-qPCR, Western blot, triglyceride detection, and Oil Red O staining assessed circMAPK9 expression and adipogenesis.
• FISH, luciferase reporter assays, and RIP verified the circMAPK9/hsa-miR-1322/FTO interaction.

Main Results:

• 164 circRNAs were differentially expressed between lean and obese individuals.
• circMAPK9 expression was significantly elevated in obese individuals and correlated with BMI.
• circMAPK9 acts as a molecular sponge for hsa-miR-1322, relieving its inhibition on FTO and promoting adipogenesis.

Conclusions:

• circMAPK9 plays a critical role in promoting adipogenesis.
• The circMAPK9/hsa-miR-1322/FTO axis represents a novel regulatory pathway in obesity.